cssS [2019-08-28 09:24:25]

two-component sensor kinase, control of cellular responses to protein secretion stress

locus: BSU_33020

pI: 5.80

mw: 51.92 kDa

protein length: 451 aa

gene length: 1356 bp

function: control of cellular responses to protein secretion stress

product: two-component sensor kinase

essential: no

synonyms: yvqB

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

Gene

Coordinates: 3,386,398 3,387,753

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of [protein|A40CD2C23A19860342F440284302EFFDAC09E88A|CssR]
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

Paralogous protein(s)

• [protein|52BB660B0CAB36C74F1D47963235846B45AF78AB|YclK], [protein|96E4470EF4B558F4EC318FEBF58B779BA50DB7E2|YvrG], [protein|9E8A6AB3920BFBFDAAD1F2E4F333A32354ABB25B|YkoH], [protein|1582E81F7DA85F38D6732D341ABD0F052587F25A|KinE]

[SW|Domains]

• two transmembrane segments, C-terminal histidine phosphotransferase domain

Modification

• autophosphorylation on a His residue

Effectors of protein activity

• the extracellular loop domain is required for signal perception [Pubmed|22307758]

[SW|Localization]

• cell membrane (according to Swiss-Prot)
• localized primarily at the division septum but also found in a punctate pattern with lower intensity throughout the cell cylinder [Pubmed|22307758]

Expression and Regulation

Operons

• genes: [gene|A40CD2C23A19860342F440284302EFFDAC09E88A|cssR]-[gene|4EE48E4931F662E51586DB6D01E91C688329222D|cssS]
  description: [Pubmed|11555295]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12270824], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|A40CD2C23A19860342F440284302EFFDAC09E88A|CssR]: activation, [Pubmed|12270824], in [regulon|A40CD2C23A19860342F440284302EFFDAC09E88A|CssR regulon]

  regulation

  • expressed under conditions of secretion stress ([protein|search|CssR]) [Pubmed|12270824]

Biological materials

Mutant

• MGNA-B216 (yvqB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1215 NBRP B. subtilis, Japan]
• BKE33020 ([gene|4EE48E4931F662E51586DB6D01E91C688329222D|cssS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATGATGACATCATCCT, downstream forward: _UP4_TAGACTGTAGATGTTTTGCA
• BKK33020 ([gene|4EE48E4931F662E51586DB6D01E91C688329222D|cssS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATGATGACATCATCCT, downstream forward: _UP4_TAGACTGTAGATGTTTTGCA

References

Reviews

• 27518094

Original Publications

• 10094672,17600057,21630458,17088376,12270824,11555295,19820159,21602801,21624103,22307758